Introduction to Nucleic Acid Protein Analyzers

Based on the successful experience of the BioPhotometer Nucleic Acid Protein Assay, Eppendorf is proud to introduce the new BioPhotometer plus Nucleic Acid Protein Assay. This compact but powerful instrument not only provides easy operation, but also provides reliable and fast detection results.

Compared to the original 12 conventional detection methods, BioPhotometer plus adds three wavelengths, enabling 32 rapid detection methods, of which 9 can be directly pressed to obtain results. The new method can detect nucleic acid and protein fluorescent dyes (such as Cy3, Cy5 labeling rate and concentration detection), enzyme activity detection, cell biology detection and single wavelength direct detection.

Biophotometer plus's exclusive blue sample slide protects against loss, sample contamination and dust. The compact size and operating mode without the need for an external computer are suitable for laboratory operation. Independent function key design directly obtains detection data and results, avoiding manual calculation; the redesigned operation panel uses different colors to distinguish function keys, and classifies different detection methods to reduce misoperation.

BioPhotometer plus universal cuvettes, such as Eppendorf UVette® plastic cuvettes, can also be matched with adapters such as Hellma® TrayCell for ultra-micro sample detection, broadening the scope of application of the instrument.

The BioPhotometer plus Nucleoprotein Analyzer is a compact UV/Vis spectrophotometer designed for the Molecular Biology, Biochemistry and Cell Biology laboratories. It offers 32 routine fast test methods, of which 9 are freely programmable.

The BioPhotometer plus is exquisitely light and compact, with a durable metal case that is easy to carry and clean. Not only can be used for the use of cuvettes (such as Eppendorf's Vette plastic cells), can also be adapted micro-cells for micro-sample detection. Just press a key to detect and display the result of the calculation. The test results and all data are displayed in full screen at a glance, ensuring safe and error-free operation.

Nucleic acid protein analyzer product features

* 9 wavelengths are suitable for routine testing in laboratories of molecular biology, biochemistry and cell biology

* Various preset methods are available, including nucleic acid and protein quantification and fluorescent dye labeling rates

* Custom detection at 340 nm, 405 nm, and 490 nm for high flexibility

* Automatically calculate the sample dilution

* Single wavelength detection without any calculation

* Save the latest 100 experimental results and all relevant data

* Simple user operation guide to avoid misuse

* Compact design, strong and durable

* It is ready to use without any warm-up

* Fast detection time

* No external computer required, single machine can operate

* Xenon light source, long life, high light intensity

Nucleic acid protein analyzer product application

Fast and reliable detection of nucleic acid, protein, cell density and fluorescent dyes in biomolecules, single-wavelength absorbance detection and endpoint detection, and other cell biology and biochemical tests.

Interpretation of several detection methods of nucleic acid protein analyzer

BioPhotometer plus Nucleoprotein Protein Analyzer Frequently Asked Questions - What is the “340 Detection Method” for?

The "340 Detection Method" is mainly used to detect the NADH enzyme reaction. The enzyme consumption was analyzed by endpoint detection. BioPhotometer plus detects NADH at 340 nm, and results are obtained by dilution, standard or standard curve.

The endpoint detection of BioPhotometer plus allows cytotoxicity testing at 490 nm. This method is suitable for the detection of vitality of living cells. Based on the lactate dehydrogenase reaction combined with a special catalyst, BioPhotometerplus detects the end product “formazan” at 490 nm. Similar to the "340 Test Method", the test results can be obtained by dilution, standard, or standard curve.

BioPhotometer plus Nucleoprotein Protein Analyzer Frequently Asked Questions - What is the “405 Detection Method” for?

This method is mainly applied to the end point detection of the enzyme consumption indicated by the artificial substrate. Artificial substrates are indicators of the activity of certain special enzymes such as beta-galactosidase or alkaline phosphatase. Enzyme degradation products are generally p-nitro or o-nitrophenol. BioPhotometer plus detects both chemicals at 405 nm. Similar to the "340 Test Method", the test results can be obtained by dilution, standard, or standard curve.

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